Title

In Vitro Analog Of Human Bone Marrow From 3D Scaffolds With Biomimetic Inverted Colloidal Crystal Geometry

Keywords

Bone marrow; Colloidal crystals; Scaffolds; Stem cell; Tissue engineering

Abstract

In vitro replicas of bone marrow can potentially provide a continuous source of blood cells for transplantation and serve as a laboratory model to examine human immune system dysfunctions and drug toxicology. Here we report the development of an in vitro artificial bone marrow based on a 3D scaffold with inverted colloidal crystal (ICC) geometry mimicking the structural topology of actual bone marrow matrix. To facilitate adhesion of cells, scaffolds were coated with a layer of transparent nanocomposite. After seeding with hematopoietic stem cells (HSCs), ICC scaffolds were capable of supporting expansion of CD34+ HSCs with B-lymphocyte differentiation. Three-dimensional organization was shown to be critical for production of B cells and antigen-specific antibodies. Functionality of bone marrow constructs was confirmed by implantation of matrices containing human CD34+ cells onto the backs of severe combined immunodeficiency (SCID) mice with subsequent generation of human immune cells. © 2008 Elsevier Ltd.

Publication Date

2-1-2009

Publication Title

Biomaterials

Volume

30

Issue

6

Number of Pages

1071-1079

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.biomaterials.2008.10.041

Socpus ID

57749190559 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/57749190559

This document is currently not available here.

Share

COinS