Title

Adult Rat Spinal Cord Culture On An Organosilane Surface In A Novel Serum-Free Medium

Keywords

Adult rat spinal cord; Cell culture; Electrophysiology; Motoneuron; Serum-free medium; Silane surface

Abstract

In this study, we have documented by morphological analysis, immunocytochemistry, and electrophysiology, the development of a culture system that promotes the growth and long-term survival of dissociated adult rat spinal cord neurons. This system comprises a patternable, nonbiological, cell growth-promoting organosilane substrate coated on a glass surface and an empirically derived novel serum-free medium, supplemented with specific growth factors (acidic fibroblast growth factor, heparin sulfate, neurotrophin-3, brain-derived neurotrophic factor, glial-derived neurotrophic factor, cardiotrophin-1, and vitronectin). Neurons were characterized by immunoreactivity for neurofilament 150, neuron-specific enolase, Islet-1 antibodies, electrophysiology, and the cultures were maintained for 4-6 wk. This culture system could be a useful tool for the study of adult mammalian spinal neurons in a functional in vitro system. © 2005 Society for In Vitro Biology.

Publication Date

11-1-2005

Publication Title

In Vitro Cellular and Developmental Biology - Animal

Volume

41

Issue

10

Number of Pages

343-348

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1290/0505031.1

Socpus ID

33645038375 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/33645038375

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