Title

Evaluation Of Two Lanthanide Complexes For Qualitative And Quantitative Analysis Of Target Proteins Via Partial Least Squares Analysis

Keywords

Carbonic anhydrase; Human serum albumin; Lanthanide ions; Luminescence; Partial least squares regression; Proteins; γ-Globulin

Abstract

Two lanthanide complexes, namely 5-aminosalicylic acid ethylenediaminetetraacetate europium(III) (5As-EDTA-Eu3+) and 4-aminosalicylic acid ethylenediaminetetraacetate terbium(III), were evaluated for the analysis of carbonic anhydrase, human serum albumin (HSA), and γ-globulin. Quantitative analysis is based on their luminescence enhancement upon protein binding and qualitative analysis on their lifetime capability to recognize the binding protein. Analytical figures of merit are presented for the three proteins. The limits of detection with 5As-EDTA-Eu 3+ are at the parts per billion level. Partial least square regression analysis is used to determine HSA and γ-globulin in binary mixtures without previous separation at the concentration ranges typically found in clinical tests of human blood serum. © 2004 Elsevier Inc. All rights reserved.

Publication Date

1-1-2005

Publication Title

Analytical Biochemistry

Volume

336

Issue

1

Number of Pages

64-74

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.ab.2004.09.020

Socpus ID

9944260806 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/9944260806

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