Title
Fluorescent Dyes For Multiphoton Bio-Imaging Applications
Keywords
Multiphoton imaging; Reactive dyes; Two-photon fluorescence
Abstract
Fluorescent dyes and probes are key components in multiphoton based fluorescence microscopy imaging of biological samples. While many commercially available fluorescent dyes have sufficed, most exhibit relatively low two-photon absorption (2PA) cross-section values in the tunability range of Tirsapphire lasers commonly used in multiphoton microscopy imaging. Furthermore, available fluorophores may be plagued with either low fluorescence quantum yields and/or the additional problem of rapid photobleaching upon exposure to the high peak powers provided by fs laser sources. In order to address the demand for better performing dyes for two-photon based imaging, we have prepared a new series of reactive fluorophores tailored for multiphoton imaging. These fluorophores are based upon the fluorene ring system, known to exhibit high fluorescence quantum yields, typically > 0.7, and possess high photostability. They have been functionalized with moieties to act, e.g., as efficient amine-reactive fluorescent probes for the covalent attachment onto, e.g., proteins and antibodies. The synthesis and the single-photon spectral characteristics, as well as measured two-photon absorption cross sections of the reactive fluorophores in solution will be presented. Spectral characterizations of bovine serum albumin (BSA) conjugated with the new reactive probe will also be presented.
Publication Date
11-16-2004
Publication Title
Proceedings of SPIE - The International Society for Optical Engineering
Volume
5329
Number of Pages
201-206
Document Type
Article; Proceedings Paper
Personal Identifier
scopus
DOI Link
https://doi.org/10.1117/12.529412
Copyright Status
Unknown
Socpus ID
7644239495 (Scopus)
Source API URL
https://api.elsevier.com/content/abstract/scopus_id/7644239495
STARS Citation
Schafer, Katherine J.; Yao, Sheng; Belfield, Kevin D.; Hales, Joel M.; and Hagan, David J., "Fluorescent Dyes For Multiphoton Bio-Imaging Applications" (2004). Scopus Export 2000s. 4969.
https://stars.library.ucf.edu/scopus2000/4969