Title

An Investigation On The Analytical Potential Of Polymerized Liposomes Bound To Lanthanide Ions For Protein Analysis

Abstract

We present a promising approach to protein sensing based on Eu3+ ions incorporated into polymerized liposomes. The sensitization of Eu 3+ is accomplished with 5-aminosalicylic acid, which provides energy transfer for a stable reference signal and a wide wavelength excitation range free from protein interference. The lipophilic character of polymerized liposomes provides the appropriate platform for protein interaction with the lanthanide ion. Quantitative analysis is based on the linear relationship between the luminescence signal of Eu3+ and protein concentration. Because no spectral shift of the lanthanide luminescence is observed upon protein interaction, qualitative analysis is based on the luminescence lifetime of polymerized liposomes. This parameter, which changes significantly upon protein-liposome interaction, follows a well-behaved single-exponential decay that might be useful for protein identification.

Publication Date

9-1-2004

Publication Title

Journal of the American Chemical Society

Volume

126

Issue

34

Number of Pages

10738-10745

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1021/ja048963b

Socpus ID

4344659703 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/4344659703

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