Title

Cloning And Heterologous Expression Of A Methanococcus Vannielii Gene Encoding A Selenium-Binding Protein

Keywords

Bound selenium; Hydrophobic protein; Immunoaffinity chromatography; Selenite; Selenium mobilization; Sequence homology

Abstract

The activation and incorporation of selenium into selenocysteine containing selenoproteins has been well established in an Escherichia coli model system but there is little specific information concerning the transport and intracellular trafficking of selenium in biological systems in general. A selenium transport role is a possible function of a novel 42 kDa selenium-binding protein that recently was purified from Methanococcus vannielii. The gene encoding a monomer of this protein (Sbp) has been cloned, sequenced and heterologously expressed in E. coli. The 8.8 kDa gene product contains 81 amino acids. The recombinant Sbp (rSbp) protein was shown to bind selenium from added selenite. The bound selenium appeared predominantly in dimeric and tetrameric forms of the protein. The gene encoding Sbp occurs in an operon that contains a carbonic anhydrase gene and selenocysteine-containing formate dehydrogenase genes, suggesting possible roles in selenium-dependent formate metabolism.

Publication Date

8-1-2004

Publication Title

IUBMB Life

Volume

56

Issue

8

Number of Pages

501-507

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1080/15216540400010818

Socpus ID

9644294205 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/9644294205

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