Title

Swgdam Developmental Validation Of A 19-Locus Y-Str System For Forensic Casework

Keywords

DYS19; DYS385; DYS388; DYS389; DYS390; DYS391; DYS392; DYS393; DYS425; DYS434; DYS437; DYS438; DYS439; Forensic science; Multiplex Y-STR analysis; Post-coital cervicovaginal samples; SWGDAM validation; Y-GATA-A7.1 (DYS460); Y-GATA-A7.2 (DYS461); Y-GATA-C4; Y-GATA-H4

Abstract

A Scientific Working Group on DNA Analysis Methods (SWGDAM) developmental validation study was carried out on two Y-STR multiplex systems (MPI and MPII) that collectively permit the co-amplification of 19 Y-STR markers, including DYS393, DYS392, DYS391, DYS389I, DYS389II, Y-GATA-A7.2 (DYS461), DYS438, DYS385a and DYS385b (MPI); DYS425, DYS388, DYS390, DYS439, DYS434, DYS437, Y-GATA-C.4, Y-GATA-A7.1 (DYS460), Y-GATA-H.4, and DYS 19 (MPII). Performance checks subsequent to PCR parameter optimization indicated that MPI and MPII were suitably reproducible, precise and accurate for forensic use. The sensitivity of the systems was such that a full 19-locus Y-STR profile was obtainable with 150-200 pg of male DNA, and some loci were detectable even with as little as 20-30 pg of input DNA. Primate specificity was demonstrated by the lack of cross-reactivity with a variety of commonly encountered bacterial and animal species, with the single exception of a monomorphic canine product that was outside of the size range of human alleles from any of the 19 loci. Not surprisingly, cross-reactivity was observed with a number of male and female nonhuman primates. Environmentally compromised samples produced full or partial Y-STR profiles. For example, a semen stain exposed to the outdoor elements for six months still gave a 13-locus Y-STR profile. Although a limited number of female DNA artifacts were observed in mixed stains in which the male DNA comprised 1/300 of the total, the full 19-locus male profile was easily discernible. Even at a 1500-to-2000-fold dilution of male DNA with female DNA partial Y-STR profiles were obtained. Furthermore, the potential utility of MPI and MPII for forensic casework is exemplified by their ability to dissect out the male haplotype in a variety of case-type samples, including, inter alia, post-coital vaginal swabs, admixed male and female bloodstains, the nonsperm fraction from a differentially extracted semen stain, and determination of the number of male donors in mixed semen stains.

Publication Date

1-1-2004

Publication Title

Journal of Forensic Sciences

Volume

49

Issue

4

Number of Pages

668-683

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1520/jfs2003134

Socpus ID

3142666966 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/3142666966

This document is currently not available here.

Share

COinS