Title

Mrna Profiling For Body Fluid Identification By Multiplex Quantitative Rt-Pcr

Keywords

Blood identification; Body fluid identification; Forensic science; Menstrual blood identification; mRNA profiling; Multiplex real-time PCR; RNA; Saliva identification; Semen identification

Abstract

An alternative approach to conventional protein-based body fluid identification is gene expression profiling analysis. In the present work, we report the development of sensitive and robust multiplex quantitative reverse transcriptase-PCR assays for the identification of blood, saliva, semen, and menstrual blood. Each body fluid assay comprises a triplex system that detects transcripts from two body fluid-specific genes and a housekeeping gene GAPDH. The body fluid-specific genes include erythroid δ-aminolevulinate synthase (ALAS2) and β-spectrin (SPTB) for blood, statherin (STATH) and histatin 3 (HTN3) for saliva, protamine 1 (PRM1) and protamine 2 (PRM2) for semen, and matrix metalloproteinase 7 (MMP7) and matrix metalloproteinase 10 (MMP10) for menstrual blood. Normalization of both body fluid-specific genes to the housekeeping gene by means of appropriate cycle threshold metrics ensures the high specificity of each assay for its cognate body fluid. © 2007 American Academy of Forensic Sciences.

Publication Date

11-1-2007

Publication Title

Journal of Forensic Sciences

Volume

52

Issue

6

Number of Pages

1252-1262

Document Type

Article; Proceedings Paper

Personal Identifier

scopus

DOI Link

https://doi.org/10.1111/j.1556-4029.2007.00550.x

Socpus ID

36949041028 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/36949041028

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