Title

Reorganization Of Mouse Sperm Lipid Rafts By Capacitation

Keywords

Capacitation; Lipid raft; Membrane organization

Abstract

One of the hallmarks of mammalian sperm capacitation is the loss of cholesterol from the plasma membrane. Cholesterol has been associated with the formation of detergent insoluble membrane microdomains in many cell types, and sperm from several mammalian species have been shown to contain detergent-resistant membranes (DRMs). The change in cholesterol composition of the sperm plasma membrane during capacitation raises the question of whether the contents of DRMs are altered during this process. In this study, we investigated changes in protein composition of DRMs isolated from uncapacitated or capacitated mouse sperm. TX-100 insoluble membranes were fractionated by sucrose flotation gradient centrifugation and analyzed by Western and lectin blotting, and capacitation-related differences in protein composition were identified. Following capacitation, the detergent insoluble fractions moved to lighter positions on the sucrose gradients, reflecting a global change in density or composition. We identified several individual proteins that either became enriched or depleted in DRM fractions following capacitation. These data suggest that the physiological changes in sperm motility, ability to penetrate the zona pellucida (ZP), ZP responsiveness, and other capacitation-dependent changes, may be due in part to a functional reorganization of plasma membrane microdomains. © 2006 Wiley-Liss, Inc.

Publication Date

12-1-2006

Publication Title

Molecular Reproduction and Development

Volume

73

Issue

12

Number of Pages

1541-1549

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1002/mrd.20540

Socpus ID

33750287154 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/33750287154

This document is currently not available here.

Share

COinS