Title

Testing And Evaluation Of 43 "Noncore" Y Chromosome Markers For Forensic Casework Applications

Keywords

Forensic science; MPIII; MPIV; MPV; Multiplex; SWGDAM validation; Y chromosome STR

Abstract

A developmental validation study was performed on three Y-STR multiplex systems, Multiplex III (MPIII), Multiplex IV (MPIV), and Multiplex V (MPV), to ascertain their potential applicability to forensic casework. MPIII contains eight Y-STRs, including DYS426, DYS435, DYS436, DYS441, DYS442, DYS446, DYS462, and Y-GATA-A10, and one InDel, YAP (DYS287). MPIV contains 21 Y-STR loci, including DYS443, DYS444, DYS445, DYS447, DYS448, DYS449, DYS452, DYS453, DYS454, DYS455, DYS456, DYS458, DYS463, DYS464, DYS468, DYS484, DYS522, DYS527, DYS531 DYS557, and DYS588. MPV contains 13 Y-STR loci, including DYS459, DYS476, DYS488, DYS513, DYS549, DYS561, DYS570, DYS575, DYS576, DYS590, DYS594, DYS598, and DYS607. Full genetic profiles were consistently obtained for all three multiplexes with 25-50 pg of male DNA. No significant amplification was observed with 1 μg of female DNA. Each multiplex permitted the determination of the number of male donors in male:male DNA admixtures. Species specificity studies demonstrated some cross-reactivity with some primate samples. Environmentally compromised blood samples produced full or partial profiles after exposure to various conditions for up to 1 year. Full profiles were recovered from simulated casework specimens including cigarette butts and postcoital cervicovaginal swabs. Population data were collected to determine individual loci gene diversity and multiplex discriminatory capacity. Copyright © 2006 by American Academy of Forensic Sciences.

Publication Date

11-1-2006

Publication Title

Journal of Forensic Sciences

Volume

51

Issue

6

Number of Pages

1298-1314

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1111/j.1556-4029.2006.00263.x

Socpus ID

33845581805 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/33845581805

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