Title

Expression Of Prostasin And Protease Nexin-1 In Rhesus Monkey (Macaca Mulatta) Endometrium And Placenta During Early Pregnancy

Keywords

Endometrium; Placenta; Pregnancy; Prostasin; Protease nexin-1; Rhesus monkey

Abstract

Serine proteases have been documented to play key roles in uterine matrix turnover and trophoblastic invasion during implantation. Roles of prostasin serine protease in these processes, however, are currently unclear. The present study was first conducted to investigate the colocalization of prostasin and its cognate serpin, protease nexin-1 (PN-1), in rhesus monkey endometrium and placenta on days 12, 18, and 26 of pregnancy by using in situ hybridization (ISH) and immunohistochemistry. With ISH, expression of prostasin mRNA was intensely localized in the glandular epithelium on days 12 and 18 and in the placental villi, trophoblastic column, trophoblastic shell, and fetal-maternal border on days 18 and 26. With the progress of pregnancy, expression level in the glandular epithelium was significantly decreased, and the accumulation in the placental compartments was further increased. In addition, the stroma and arterioles exhibited modest levels of prostasin signals. However, expression level of PN-1 in these compartments on adjacent sections in the three stages of early pregnancy was weak or below the level of detection. Prostasin protein expression in the endometrium was found to be consistent with the distribution patterns revealed in the ISH experiments. It may be suggested from these results that prostasin is involved in endometrial epithelial morphology establishment, tissue remodeling, and trophoblastic invasion during early pregnancy. The cognate serpin PN-1 was not coordinately expressed along with prostasin, creating a tissue environment favorable for proteolytic activities of prostasin during early pregnancy events. © The Histochemical Society, Inc.

Publication Date

10-1-2006

Publication Title

Journal of Histochemistry and Cytochemistry

Volume

54

Issue

10

Number of Pages

1139-1147

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1369/jhc.6A7005.2006

Socpus ID

33748939245 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/33748939245

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