Title

Identification Of A Diacylglycerol Acyltransferase Gene Involved In Accumulation Of Triacylglycerol In Mycobacterium Tuberculosis Under Stress

Abstract

Mycobacterium tuberculosis under stress stores triacylglycerol (TG). There are 15 genes in M. tuberculosis that belong to a novel family of TG synthase genes (tgs), but it is not known which of them is responsible for this accumulation of TG. In this paper, it is reported that M. tuberculosis H37Rv accumulated TG under acidic, static or hypoxic growth conditions, or upon treatment with NO, whereas TG accumulation was drastically reduced in the tgs1 (Rv3130c) disrupted mutant. Complementation with tgs1 restored this TG accumulation. C26 was a major fatty acid in this TG, indicating that the TGS1 gene product uses C26 fatty acid, which is known to be produced by the mycobacterial fatty acid synthase. TGS1 expressed in Escherichia coli preferred C26:0 CoA for TG synthesis. If TG storage is needed for the long-term survival of M. tuberculosis under dormant conditions, the tgs1 product could be a suitable target for antilatency drugs. © 2006 SGM.

Publication Date

9-1-2006

Publication Title

Microbiology

Volume

152

Issue

9

Number of Pages

2717-2725

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1099/mic.0.28993-0

Socpus ID

33748769518 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/33748769518

This document is currently not available here.

Share

COinS