Title

Interference Of Egfp Rna In Human Nt-2/D1 Cell Lines Using Human U6 Promoter-Based Sirna Pcr Products

Keywords

Gene silencing; Human teratocarcinoma NT-2/D1 cells; PCR; Small interference RNA

Abstract

RNA interference (RNAi), a process of sequence-specific gene suppression, has been known as a natural gene regulatory mechanism in a wide range of lower organisms. Recently, we have reported that a transfection of human U6 promoter (hU6) driven hairpin small-interference RNA (siRNA) plasmid specifically knocks down the target gene by post-transcriptional gene silencing in mammalian cells. Here we report that transfection of polymerase chain reaction (PCR) products, containing human U6 promoter with hairpin siRNA, knocks down the target gene expression in human teratocarcinoma NT-2/D1 cells. Moreover, we showed 3′ end termination sequence, 5 Ts, is not critical elements for knocking down in PCR-based siRNA system. Therefore, the PCR-based siRNA system is a promising tool not only for the screening but also to temporally regulate gene expression in the human progenitor cells. ©KSBB.

Publication Date

5-1-2006

Publication Title

Biotechnology and Bioprocess Engineering

Volume

11

Issue

3

Number of Pages

273-276

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1007/BF02932043

Socpus ID

33745585084 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/33745585084

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