Title

Ultrahigh Resolution Crystal Structures Of Human Carbonic Anhydrases I And Ii Complexed With "Two-Prong" Inhibitors Reveal The Molecular Basis Of High Affinity

Abstract

The atomic-resolution crystal structures of human carbonic anhydrases I and II complexed with "two-prong" inhibitors are reported. Each inhibitor contains a benzenesulfonamide prong and a cupric iminodiacetate (IDA-Cu 2+) prong separated by linkers of different lengths and compositions. The ionized NH- group of each benzenesulfonamide coordinates to the active site Zn2+ ion; the IDA-Cu2+ prong of the tightest-binding inhibitor, BR30, binds to H64 of CAII and H200 of CAI. This work provides the first evidence verifying the structural basis of nanomolar affinity measured for two-prong inhibitors targeting the carbonic anhydrases. © 2006 American Chemical Society.

Publication Date

3-8-2006

Publication Title

Journal of the American Chemical Society

Volume

128

Issue

9

Number of Pages

3011-3018

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1021/ja057257n

Socpus ID

33644956925 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/33644956925

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