Title
Assessing Mitochondrial Outer Membrane Permeabilization During Apoptosis
Keywords
Bax and t-Bid; Cell death; Cytochrome c; Fluorescence microscopy; Mitochondrial outer membrane permeability
Abstract
Mitochondria play a pivotal role in the regulation of apoptosis. An imbalance in apoptosis can lead to disease. Unscheduled apoptosis has been linked to neurodegeneration while inhibition of apoptosis can cause cancer. An early and key event during apoptosis is the release of factors from mitochondria. In apoptosis the mitochondrial outer membrane becomes permeable, leading to release of apoptogenic factors into the cytosol. One such factor, cytochrome c, is an electron carrier of the respiratory chain normally trapped within the mitochondrial intermembrane space. Many apoptotic studies investigate mitochondrial outer membrane permeabilization (MOMP) by monitoring the release of cytochrome c. Here, we describe three reliable techniques that detect cytochrome c release from mitochondria, through subcellular fractionation or immunocytochemistry and fluorescence microscopy, or isolated mitochondria and recombinant Bax and t-Bid proteins in vitro. These techniques will help to identify mechanisms and characterize factors regulating MOMP. © 2008.
Publication Date
12-1-2008
Publication Title
Methods
Volume
46
Issue
4
Number of Pages
319-323
Document Type
Article
Personal Identifier
scopus
DOI Link
https://doi.org/10.1016/j.ymeth.2008.10.019
Copyright Status
Unknown
Socpus ID
56249101739 (Scopus)
Source API URL
https://api.elsevier.com/content/abstract/scopus_id/56249101739
STARS Citation
Dave, Zankruti; Byfield, Maya; and Bossy-Wetzel, Ella, "Assessing Mitochondrial Outer Membrane Permeabilization During Apoptosis" (2008). Scopus Export 2000s. 9250.
https://stars.library.ucf.edu/scopus2000/9250