Title

Assessing Mitochondrial Morphology And Dynamics Using Fluorescence Wide-Field Microscopy And 3D Image Processing

Keywords

Astrocytes; Fluorescence widefield microscopy; Mitochondrial fission; Mitochondrial fusion; Mitochondrial length; Mitochondrial number; Motor neurons; Time-lapse imaging

Abstract

Mitochondrial morphology and length change during fission and fusion and mitochondrial movement varies dependent upon the cell type and the physiological conditions. Here, we describe fundamental wide-field fluorescence microcopy and 3D imaging techniques to assess mitochondrial shape, number and length in various cell types including cancer cell lines, motor neurons and astrocytes. Furthermore, we illustrate how to assess mitochondrial fission and fusion events by 3D time-lapse imaging and to calculate mitochondrial length and numbers as a function of time. These imaging methods provide useful tools to investigate mitochondrial dynamics in health, aging and disease. © 2008 Elsevier Inc. All rights reserved.

Publication Date

12-1-2008

Publication Title

Methods

Volume

46

Issue

4

Number of Pages

295-303

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.ymeth.2008.10.003

Socpus ID

56249089371 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/56249089371

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