Title

Growth And Electrophysiological Properties Of Rat Embryonic Cardiomyocytes On Hydroxyl- And Carboxyl-Modified Surfaces

Keywords

Carboxyl; Cardiac tissue engineering; Cardiomyocytes; Cell culture; Electrophysiology; Hydroxyl; PLA; PLGA; SAM; Scaffolds; Serum-free

Abstract

Biodegradable scaffolds such as poly(lactic acid) (PLA), poly(lactic-co-glycolic acid) (PLGA) or poly(glycolic acid) (PGA) are commonly used materials in tissue engineering. The chemical composition of these scaffolds changes during degradation which provides a differential environment for the seeded cells. In this study we have developed a simple and relatively high-throughput method in order to test the physiological effects of this varying chemical environment on rat embryonic cardiac myocytes. In order to model the different degradation stages of the scaffold, glass coverslips were functionalized with 11-mercaptoundecanoic acid (MUA) and 11-mercapto-1-undecanol (MUL) as carboxyl- and hydroxyl-groups presenting surfaces, and with trimethoxysilylpropyldiethylenetriamine (DETA) and (3-aminopropyl) triethoxysilane (APTES) as controls. Embryonic cardiac myocytes formed beating islands on all tested surfaces, but the number of attached cells and beating patches was significantly lower on MUL compared to any of the other functionalized surfaces. Moreover, whole-cell patch-clamp experiments showed that the average length of action potentials generated by the beating-cardiac myocytes were significantly longer on MUL compared to the other surfaces. Our results, using our simple test system, are in basic agreement with earlier observations that utilized a complex 3D biodegradable scaffold. Thus, surface functionalization with self-assembled monolayers combined with histological/physiological testing could be a relatively high throughput method for biocompatibility studies and for the optimization of the material/tissue interface in tissue engineering. © 2008 VSP.

Publication Date

10-1-2008

Publication Title

Journal of Biomaterials Science, Polymer Edition

Volume

19

Issue

10

Number of Pages

1319-1331

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1163/156856208786052399

Socpus ID

61849135431 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/61849135431

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