Title

Sub-Cellular Resolution Imaging With Gabor Domain Optical Coherence Microscopy

Keywords

Adaptive imaging; Microscopy; Optical coherence tomography; Variable focus microscope objective

Abstract

Optical Coherence Microscopy (OCM) utilizes a high NA microscope objective in the sample arm to achieve an axially and laterally high resolution OCT image. An increase in NA, however, leads to a dramatically decreased depth of focus (DOF), and hence shortens the imaging depth range so that high lateral resolution is maintained only within a small depth region around the focal plane. One solution to increase the depth of imaging while keeping a high lateral resolution is dynamic-focusing. Utilizing the voltage controlled refocus capability of a liquid lens, we have recently presented a solution for invariant high resolution imaging using the liquid lens embedded within a fixed optics hand-held custom microscope designed specifically for optical imaging systems using a broadband light source at 800 nm center wavelength. Subsequently, we have developed a Gabor-Domain Optical Coherence Microscopy (GD-OCM) that utilizes the high speed imaging of spectral domain OCT, the high lateral resolution of OCM, and the ability of real time refocusing of our custom design variable focus objective. In this paper we demonstrate in detail how portions of the infocus cross-sectional images can be extracted and fused to form an invariant lateral resolution image with multiple crosssectional images acquired corresponding to a discrete refocusing step along depth enabled by the varifocal probe. We demonstrate sub-cellular resolution imaging of an African frog tadpole (Xenopus Laevis) taken from a 500 μm x 500 μm cross-section. © 2010 Copyright SPIE - The International Society for Optical Engineering.

Publication Date

5-3-2010

Publication Title

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Volume

7554

Number of Pages

-

Document Type

Article; Proceedings Paper

Personal Identifier

scopus

DOI Link

https://doi.org/10.1117/12.842662

Socpus ID

77951584235 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/77951584235

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