Title

An Immunoassay For Monoclonalantibody Isotyping And Quality Analysis Using Gold Nanoparticle Probes And Dynamic Light Scattering

Abstract

A convenient, easy-to-use, and economical immunoassay for analysis and direct monitoring of antibody-antigen binding was demonstrated. As an example, the assay was used for MAb isotype and quality analysis. Among the 10 MAbs tested, all four from vendor A showed high quality, while among the six anticancer biomarker MAbs received from vendor B, two had quality problems and one had incorrect isotype information provided by the company. Using the NanoDL-Say assay, one can conveniently check the quality and other information about antibody product used for research and development. DLS is an inexpensive (a basic unit costs $20,000-40,000), user-friendly, and virtually maintenance-free instrument, easily affordable to most research laboratories. The preparation of gold nanoparticle bioconjugate has been very well developed, and such conjugates can be purchased from many commercial sources or easily prepared in-house using a simple, almost labor-free procedure. The calculated cost for each test using NanoDLSay is around $.20-30 or less, while the commercial assay kit costs $20 per test. NanoDLSay is extremely easy to conduct and results can be obtained within a few minutes. Unlike ELISA or a lateral flow immunoassay test, the binding kinetics and affinity of analytes to the nanoparticle probes can be monitored in situ by NanoDLSay. More importantly, the assay can easily be extended to the analysis and interaction study of other biomolecules, as long as the target biomolecule has a size exceeding the detection limit of a DLS instrument. The assay has been used successfully to monitor protein A-IgG binding,5 avidin-biotin interaction, and many other biomolecular systems. NanoDLSay has the potential to replace or supplement SPR as a highly convenient and powerful tool for biomolecular analysis and interaction study.

Publication Date

3-1-2010

Publication Title

American Biotechnology Laboratory

Volume

28

Issue

3

Number of Pages

8-12

Document Type

Article

Personal Identifier

scopus

Socpus ID

77951875497 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/77951875497

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