Title

A Nanochannel Array-Based Electrochemical Device For Quantitative Label-Free Dna Analysis

Keywords

DNA; Electrochemical analysis; Label-free sensor; Morpholino; Nanochannel array; Porous anodic alumina membrane

Abstract

A strategy for label-free oligonucleotide (DNA) analysis has been proposed by measuring the DNA-morpholino hybridization hindered diffusion flux of probe ions Fe(CN)63- through nanochannels of a porous anodic alumina (PAA) membrane. The flux of Fe(CN)63- passing through the PAA nanochannels is recorded using an Au film electrochemical detector sputtered at the end of the nanochannels. Hybridization of the end-tethered morpholino in the nanochannel with DNA forms a negatively charged DNA-morpholino complex, which hinders the diffusion of Fe(CN)63- through the nanochannels and results in a decreased flux. This flux is strongly dependent on ionic strength, nanochannel aperture, and target DNA concentration, which indicates a synergetic effect of steric and electrostatic repulsion effects in the confined nanochannels. Further comparison of the probe flux with different charge passing through the nanochannels confirms that the electrostatic effect between the probe ions and DNA dominates the hindered diffusion process. Under optimal conditions, the present nanochannel array-based DNA biosensor gives a detection limit of 0.1 nM. © 2010 American Chemical Society.

Publication Date

11-23-2010

Publication Title

ACS Nano

Volume

4

Issue

11

Number of Pages

6417-6424

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1021/nn101050r

Socpus ID

78649611228 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/78649611228

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