Title

Hydrolysis Of Dna And Its Molecular Components In The Dry State

Keywords

Depurination; Dry state DNA; Hydrolysis of DNA

Abstract

The biochemistry of dry state DNA is of interest to the field of forensic biology. The precise chemical nature of the hydrolytic degradation products of the DNA molecule in the dry state has not been previously investigated in detail. In this study we found that the mechanistic chemistry of DNA hydrolysis appears to be the same for the hydrated and dry states. The thermodynamic parameters are also similar in both states and the activation energies for base hydrolysis are indistinguishable. The principal difference between the two states is the rate at which hydrolytic degradation occurs. The duplex configuration of dry state DNA offers much more protection for the molecule than is offered in single strand and nucleotide species. Single strand breaks of dry state duplex DNA occur with a half life of 24 ± 2 days at 65 °C and appear to occur in a mechanistic manner. © 2009 Elsevier Ireland Ltd. All rights reserved.

Publication Date

4-1-2010

Publication Title

Forensic Science International: Genetics

Volume

4

Issue

3

Number of Pages

168-177

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.fsigen.2009.08.007

Socpus ID

77649179866 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/77649179866

This document is currently not available here.

Share

COinS