Title

Lacz Reporter System For Use In Borrelia Burgdorferi

Abstract

Regulation of gene expression is critical for the ability of Borrelia burgdorferi to adapt to different environments during its natural infectious cycle. Reporter genes have been used successfully to study gene regulation in multiple organisms. We have introduced a lacZ gene into B. burgdorferi, and we show that B. burgdorferi produces a protein with detectable β-galactosidase activity in both liquid and solid media when lacZ is expressed from a constitutive promoter. Furthermore, when lacZ is expressed from the ospC promoter, β-galactosidase activity is detected only in B. burgdorferi clones that express ospC, and it accurately monitors endogenous gene expression. The addition of lacZ to the repertoire of genetic tools available for use in B. burgdorferi should contribute to a better understanding of how B. burgdorferi gene expression is regulated during the infectious cycle. © 2010, American Society for Microbiology.

Publication Date

11-1-2010

Publication Title

Applied and Environmental Microbiology

Volume

76

Issue

22

Number of Pages

7407-7412

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1128/AEM.01389-10

Socpus ID

78649714232 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/78649714232

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