Title

A Host-Specific Factor Is Necessary For Efficient Folding Of The Autotransporter Plasmid-Encoded Toxin

Keywords

Autotransporter; Circular dichroism; Plasmid-encoded toxin; Protein folding

Abstract

Autotransporters are the most common virulence factors secreted from Gram-negative pathogens. Until recently, autotransporter folding and outer membrane translocation were thought to be self-mediated events that did not require accessory factors. Here, we report that two variants of the autotransporter plasmid-encoded toxin are secreted by a lab strain of Escherichia coli. Biophysical analysis and cell-based toxicity assays demonstrated that only one of the two variants was in a folded, active conformation. The misfolded variant was not produced by a pathogenic strain of enteroaggregative E. coli and did not result from protein overproduction in the lab strain of E. coli. Our data suggest a host-specific factor is required for efficient folding of plasmid-encoded toxin. © 2009 Elsevier Masson SAS. All rights reserved.

Publication Date

2-1-2010

Publication Title

Biochimie

Volume

92

Issue

2

Number of Pages

171-177

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.biochi.2009.11.006

Socpus ID

74249112624 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/74249112624

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