Title

Thermally Addressed Immunosorbent Assay For Multiplexed Protein Detections Using Phase Change Nanoparticles

Abstract

Thermally addressed immunoassay is developed to detect multiple proteins using phase change nanoparticles as thermal barcodes. The solid to liquid phase changes of nanoparticles absorb heat energy and generate sharp melting peaks, which are used as thermal signatures to determine the existence and concentration of proteins. Multiple proteins can be detected by using different types of nanoparticles in order to create a one-to-one correspondence between one type of nanoparticle and one type of protein. The fusion enthalpy that is proportional to the amount of phase change materials has been used to derive the amount of protein. The melting temperatures of nanoparticles are designed to be higher than 100 ° C to avoid interference from species contained in the fluid. Thus, the use of thermal nanoparticles allows the detection of multiple low concentration proteins in a complex fluid such as cell lysate regardless of the color, salt concentration, and conductivity of the sample. © 2010 American Chemical Society.

Publication Date

2-15-2010

Publication Title

Analytical Chemistry

Volume

82

Issue

4

Number of Pages

1186-1190

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1021/ac902724y

Socpus ID

76849098143 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/76849098143

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