Title
Es Cells Overexpressing Microrna-1 Attenuate Apoptosis In The Injured Myocardium
Keywords
Akt; Apoptosis; Heart; MicroRNA; PTEN
Abstract
MicroRNAs (miRs) are small, single-stranded, noncoding RNA's involved in post-transcriptional negative gene regulation. Recent investigations have underscored the integral role of miRs in various biological processes including innate immunity, cell-cycle regulation, metabolism, differentiation, and cell death. In the present study, we overexpressed miR-1, a muscle-specific miR, in embryonic stem cells (miR-1-ES cells), transplanted them into the infarcted myocardium, and evaluated their impact on cardiac apoptosis and function. We provide evidence demonstrating reduced apoptosis following transplantation of miR-1-ES cells 4 weeks post-myocardial infarction as compared to respective controls assessed by TUNEL staining and a capsase-3 activity assay. Moreover, we show significant elevation in p-Akt levels and diminished PTEN levels in hearts transplanted with miR-1-ES cells as determined by enzyme-linked immunoassays. Finally, using echocardiography, we reveal mice receiving miR-1-ES cell transplantation post-myocardial infarction had significantly improved fractional shortening and ejection fraction compared with respective controls. Our data suggest transplanted miR-1-ES cells inhibit apoptosis, mediated through the PTEN/Akt pathway, leading to improved cardiac function in the infarcted myocardium. © Springer Science+Business Media, LLC. 2011.
Publication Date
11-1-2011
Publication Title
Molecular and Cellular Biochemistry
Volume
357
Issue
1-2
Number of Pages
135-141
Document Type
Article
Personal Identifier
scopus
DOI Link
https://doi.org/10.1007/s11010-011-0883-5
Copyright Status
Unknown
Socpus ID
84857375050 (Scopus)
Source API URL
https://api.elsevier.com/content/abstract/scopus_id/84857375050
STARS Citation
Glass, Carley and Singla, Dinender K., "Es Cells Overexpressing Microrna-1 Attenuate Apoptosis In The Injured Myocardium" (2011). Scopus Export 2010-2014. 1892.
https://stars.library.ucf.edu/scopus2010/1892