Title

Es Cells Overexpressing Microrna-1 Attenuate Apoptosis In The Injured Myocardium

Keywords

Akt; Apoptosis; Heart; MicroRNA; PTEN

Abstract

MicroRNAs (miRs) are small, single-stranded, noncoding RNA's involved in post-transcriptional negative gene regulation. Recent investigations have underscored the integral role of miRs in various biological processes including innate immunity, cell-cycle regulation, metabolism, differentiation, and cell death. In the present study, we overexpressed miR-1, a muscle-specific miR, in embryonic stem cells (miR-1-ES cells), transplanted them into the infarcted myocardium, and evaluated their impact on cardiac apoptosis and function. We provide evidence demonstrating reduced apoptosis following transplantation of miR-1-ES cells 4 weeks post-myocardial infarction as compared to respective controls assessed by TUNEL staining and a capsase-3 activity assay. Moreover, we show significant elevation in p-Akt levels and diminished PTEN levels in hearts transplanted with miR-1-ES cells as determined by enzyme-linked immunoassays. Finally, using echocardiography, we reveal mice receiving miR-1-ES cell transplantation post-myocardial infarction had significantly improved fractional shortening and ejection fraction compared with respective controls. Our data suggest transplanted miR-1-ES cells inhibit apoptosis, mediated through the PTEN/Akt pathway, leading to improved cardiac function in the infarcted myocardium. © Springer Science+Business Media, LLC. 2011.

Publication Date

11-1-2011

Publication Title

Molecular and Cellular Biochemistry

Volume

357

Issue

1-2

Number of Pages

135-141

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1007/s11010-011-0883-5

Socpus ID

84857375050 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84857375050

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