Title
Optimization Of Dried Stain Co-Extraction Methods For Efficient Recovery Of High Quality Dna And Rna For Forensic Analysis
Keywords
DNA/RNA co-extraction; Forensic science; MRNA profiling
Abstract
A requirement for the routine implementation of mRNA profiling methods is the ability to co-extract RNA and DNA from the same biological stain. While methodologies exist for the co-isolation of DNA and RNA from biological material, few studies include a thorough evaluation of these methods for use with forensic samples. Frequently, modifications to standard methods need to be implemented in order to accommodate the challenging nature of forensic biological samples. We therefore evaluated several co-extraction methods for ease of use and ability to recover DNA and RNA of sufficient quantity and quality for forensic analysis. From the five methods evaluated, two methods were selected for subsequent optimization (NCFS organic co-extraction and the Qiagen AllPrep DNA/RNA Mini kit). Modification of the incubation temperature and time and the inclusion of additional purification steps were found to improve the efficacy of nucleic acid recovery. © 2011 Elsevier Ireland Ltd.
Publication Date
12-1-2011
Publication Title
Forensic Science International: Genetics Supplement Series
Volume
3
Issue
1
Number of Pages
-
Document Type
Article
Personal Identifier
scopus
DOI Link
https://doi.org/10.1016/j.fsigss.2011.09.017
Copyright Status
Unknown
Socpus ID
82455167921 (Scopus)
Source API URL
https://api.elsevier.com/content/abstract/scopus_id/82455167921
STARS Citation
Parker, C.; Hanson, E.; and Ballantyne, J., "Optimization Of Dried Stain Co-Extraction Methods For Efficient Recovery Of High Quality Dna And Rna For Forensic Analysis" (2011). Scopus Export 2010-2014. 2298.
https://stars.library.ucf.edu/scopus2010/2298