Title

Optimization Of Dried Stain Co-Extraction Methods For Efficient Recovery Of High Quality Dna And Rna For Forensic Analysis

Keywords

DNA/RNA co-extraction; Forensic science; MRNA profiling

Abstract

A requirement for the routine implementation of mRNA profiling methods is the ability to co-extract RNA and DNA from the same biological stain. While methodologies exist for the co-isolation of DNA and RNA from biological material, few studies include a thorough evaluation of these methods for use with forensic samples. Frequently, modifications to standard methods need to be implemented in order to accommodate the challenging nature of forensic biological samples. We therefore evaluated several co-extraction methods for ease of use and ability to recover DNA and RNA of sufficient quantity and quality for forensic analysis. From the five methods evaluated, two methods were selected for subsequent optimization (NCFS organic co-extraction and the Qiagen AllPrep DNA/RNA Mini kit). Modification of the incubation temperature and time and the inclusion of additional purification steps were found to improve the efficacy of nucleic acid recovery. © 2011 Elsevier Ireland Ltd.

Publication Date

12-1-2011

Publication Title

Forensic Science International: Genetics Supplement Series

Volume

3

Issue

1

Number of Pages

-

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.fsigss.2011.09.017

Socpus ID

82455167921 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/82455167921

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