Title
Key Regulators Of Mitochondrial Biogenesis Are Increased In Kidneys Of Growth Hormone Receptor Knockout (Ghrko) Mice
Keywords
Calorie restriction; Growth hormone receptor knockout (GHRKO) mice; Kidneys; Mitochondrial biogenesis; Proteins; Skeletal muscles; Visceral fat
Abstract
The growth hormone receptor knockout (GHRKO) mice are remarkably long-lived and highly insulin sensitive. Alterations in mitochondrial biogenesis are associated with aging and various metabolic derangements. We have previously demonstrated increased gene expression of key regulators of mitochondriogenesis in kidneys, hearts and skeletal muscles of GHRKO mice. The aim of the present study was to quantify the protein levels of the following regulators of mitochondriogenesis: peroxisome proliferator-activated receptor γ co-activator 1α (PGC-1α), AMP-activated protein kinase α (AMPKα), phospho-AMPKα (p-AMPKα), sirtuin-3 (SIRT-3), endothelial nitric oxide synthase (eNOS), phospho-eNOS (p-eNOS), nuclear respiratory factor-1 (NRF-1) and mitofusin-2 (MFN-2) in skeletal muscles and kidneys of GHRKOs in comparison to normal mice. We also were interested in the effects of calorie restriction (CR) and visceral fat removal (VFR) on these parameters. Both CR and VFR improve insulin sensitivity and can extend life span. Results: The renal levels of PGC-1α, AMPKα, p-AMPKα, SIRT-3, eNOS, p-eNOS and MFN-2 were increased in GHRKOs. In the GHRKO skeletal muscles, only MFN-2 was increased. Levels of the examined proteins were not affected by CR (except for PGC-1α and p-eNOS in skeletal muscles) or VFR. Conclusion: GHRKO mice have increased renal protein levels of key regulators of mitochondriogenesis, and this may contribute to increased longevity of these knockouts. © 2011 John Wiley & Sons, Ltd.
Publication Date
8-1-2011
Publication Title
Cell Biochemistry and Function
Volume
29
Issue
6
Number of Pages
459-467
Document Type
Article
Personal Identifier
scopus
DOI Link
https://doi.org/10.1002/cbf.1773
Copyright Status
Unknown
Socpus ID
80051672158 (Scopus)
Source API URL
https://api.elsevier.com/content/abstract/scopus_id/80051672158
STARS Citation
Gesing, Adam; Bartke, Andrzej; Wang, Feiya; Karbownik-Lewinska, Malgorzata; and Masternak, Michal M., "Key Regulators Of Mitochondrial Biogenesis Are Increased In Kidneys Of Growth Hormone Receptor Knockout (Ghrko) Mice" (2011). Scopus Export 2010-2014. 2664.
https://stars.library.ucf.edu/scopus2010/2664