Title

Analysis Of Co-Eluted Isomers Of High-Molecular Weight Polycyclic Aromatic Hydrocarbons In High Performance Liquid Chromatography Fractions Via Solid-Phase Nanoextraction And Time-Resolved Shpol'Skii Spectroscopy

Keywords

Gold nanoparticles; High-molecular weight polycyclic aromatic hydrocarbons; Laser-excited time-resolved Shpol'skii spectroscopy; Shpol'skii spectroscopy; Solid-phase nanoextraction

Abstract

We present an accurate method for the determination of isomers of high-molecular weight polycyclic aromatic hydrocarbons co-eluted in HPLC fractions. The feasibility of this approach is demonstrated with two isomers of molecular weight 302 with identical mass fragmentation patterns, namely dibenzo[a,i]pyrene and naphtho[2,3-a]pyrene. Qualitative and quantitative analysis is carried out via laser-excited time-resolved Shpol'skii spectroscopy at liquid helium temperature. Unambiguous identification of co-eluted isomers is based on their characteristic 4.2K line-narrowed spectra in n-octane as well as their fluorescence lifetimes. Pre-concentration of HPLC fractions prior to spectroscopic analysis is performed with the aid of gold nanoparticles via an environmentally friendly procedure. In addition to the two co-eluted isomers, the analytical figures of merit of the entire procedure were evaluated with dibenzo[a,l]pyrene, dibenzo[a,h]pyrene and dibenzo[a,e]pyrene. The analytical recoveries from drinking water samples varied between 98.2±5.5 (dibenzo[a,l]pyrene) and 102.7±3.2% (dibenzo[a,i]pyrene). The limits of detection ranged from 51.1ngL -1 (naphtho[2,3-a]pyrene) to 154ngL -1 (dibenzo[a,e]pyrene). The excellent analytical figures of merit associated to its HPLC compatibility makes this approach an attractive alternative for the analysis of co-eluted isomers with identical mass spectra. © 2011 Elsevier B.V.

Publication Date

9-28-2011

Publication Title

Journal of Chromatography A

Volume

1218

Issue

39

Number of Pages

6922-6929

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.chroma.2011.08.015

Socpus ID

80052330618 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/80052330618

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