Title

Two-Photon Fluorescence Vascular Bioimaging With New Bioconjugate Probes Selective Toward The Vascular Endothelial Growth Factor Receptor 2

Abstract

We report the synthesis and characterization of two amine reactive fluorescent dyes with efficient two-photon absorption (2PA) properties and high fluorescence quantum yields. Bioconjugation of these dyes with the DC-101 antibody proved to be useful for selectively imaging the vascular endothelial growth factor receptor 2 (VEGFR-2) in cells expressing this receptor in vitro and in "whole" mounted excised tumors (ex vivo) by two-photon fluorescence microscopy (2PFM). The penetration depths reached within the tumors by 2PFM was over 800 μm. In addition, the concentration of dye required for incubation of these bioconjugates was in the picomolar domain, the probes possessed very good photostability, and the 2PFM setup did not require any additional means of increasing the collection efficiencies of fluorescent photons to achieve the relatively deep tissue imaging that was realized, due, in large part, to the favorable photophysical properties of the new probes. © 2011 American Chemical Society.

Publication Date

10-19-2011

Publication Title

Bioconjugate Chemistry

Volume

22

Issue

10

Number of Pages

2060-2071

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1021/bc200299z

Socpus ID

80054797649 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/80054797649

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