Title

The Application Of The Chloroplast Genome Of Oncidium In Plant Identification And Breeding In Oncidiinae

Abstract

Oncidium spp. is an important orchid species among cut flowers. However, determining their phylogenetic relationships and identifying varieties are very difficult. Molecular markers can provide useful tools for phylogenetic resolution, the chloroplast being one resource for those markers. The complete chloroplast genome of Onc. Gower Ramsey (Accession no. GQ324949) was determined. The size of the Oncidium chloroplast genome is 146,484 bp. Interesting, Onc. Gower Ramsey chloroplast encoded NADH dehydrogenase (ndh) genes, except ndhE, have no functions. Based on this information, eight regions of the Onc. Gower Ramsey chloroplast genome were amplified by polymerase chain reaction for phylogenetic analysis to identify the relationships between the 15 Oncidiinae hybrids. The results confirm that the chloroplast genome provides a useful molecular marker for species identification.

Publication Date

1-1-2011

Publication Title

Orchid Biotechnology II

Number of Pages

253-266

Document Type

Article; Book Chapter

Personal Identifier

scopus

DOI Link

https://doi.org/10.1142/9789814327930_0013

Socpus ID

84974603258 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84974603258

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