Title

Ubiquitin Proteasome System And The Atypical Kinase Pfpk7 Are Involved In Melatonin Signaling In Plasmodium Falciparum

Keywords

kinase; malaria; melatonin; signaling; ubiquitin

Abstract

We previously reported that melatonin modulates the Plasmodium falciparum erythrocytic cycle by increasing schizont stage population as well as diminishing ring stage population. In addition, the importance of calcium and cAMP in melatonin signaling pathway in P. falciparum was also demonstrated. Nevertheless, the molecular effectors of the indoleamine signaling pathway remain elusive. We now demonstrate by real-time PCR that melatonin treatment up-regulates genes related to ubiquitin/proteasome system (UPS) components and that luzindole, a melatonin receptor antagonist, inhibits UPS transcription modulation. We also show that protein kinase PfPK7, a P. falciparum orphan kinase, plays a crucial role in the melatonin transduction pathway, since following melatonin treatment of P. falciparum parasites where pfpk7 gene is disrupted (pfpk7- parasites) (i) the ratio of asexual stages remain unchanged, (ii) the increase in cytoplasmatic calcium in response to melatonin was strongly diminished and (iii) up-regulation of UPS genes did not occur. The wild-type melatonin-induced alterations in cell cycle features, calcium rise and UPS gene transcription were restored by re-introduction of a functional copy of the pfpk7 gene in the pfpk7- parasites. © 2012 John Wiley & Sons A/S.

Publication Date

9-1-2012

Publication Title

Journal of Pineal Research

Volume

53

Issue

2

Number of Pages

147-153

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1111/j.1600-079X.2012.00981.x

Socpus ID

84865016293 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84865016293

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