Title

X-Ray Enabled Detection And Eradication Of Circulating Tumor Cells With Nanoparticles

Keywords

Capture; Circulating tumor cells; Detection; Localization; Nanoparticles

Abstract

The early detection and eradication of circulating tumor cells (CTCs) play an important role in cancer metastasis management. This paper describes a new nanoparticle-enabled technique for integrated enrichment, detection and killing of CTCs by using magnetic nanoparticles and bismuth nanoparticles, X-ray fluorescence spectrometry, and X-ray radiation. The nanoparticles are modified with tumor targeting agents and conjugated with tumor cells through folate receptors over-expressed on cancer cells. A permanent micro-magnet is used to collect CTCs suspended inside a flowing medium that contains phosphate buffered saline (PBS) or whole blood. The characteristic X-ray emissions from collected bismuth nanoparticles, upon excitation with collimated X-rays, are used to detect CTCs. Results show that the method is capable of selectively detecting CTCs at concentrations ranging from 100-100,000. cells/mL in the buffer solution, with a detection limit of ~100. CTCs/mL. Moreover, the dose of primary X-rays can be enhanced to kill the localized CTCs by radiation induced DNA damage, with minimal invasiveness, thus making in vivo personalized CTC management possible. © 2012 Elsevier B.V.

Publication Date

10-1-2012

Publication Title

Biosensors and Bioelectronics

Volume

38

Issue

1

Number of Pages

348-354

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.bios.2012.06.020

Socpus ID

84864391593 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84864391593

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