Title

Rational Design Of Competitive Electrocatalysts For Hydrogen Fuel Cells

Keywords

Anticancer compounds; DNA binding proteins; Eukaryotic topoisomerase I; High-throughput screening; Solid phase assays

Abstract

We report a mechanism-based screening technique to rapidly identify eukaryotic topoisomerase I targeting agents. The method is based on genetic tagging of topoisomerase I to immobilize the enzyme on a solid surface in a microtiter well format. DNA is added to the wells, and retained DNA is detected by Pico Green fluorescence. Compounds that result in an increase in Pico Green staining represent potential topoisomerase interfacial poisons, whereas those that reduce fluorescence report catalytic inhibitors; therefore, the solid phase assay represents a "bimodal" readout that reveals mechanisms of action. The method has been demonstrated to work with known interfacial poisons and catalytic inhibitors. This method is rapid, robust, economical, and scalable for large library screens. © 2011 Elsevier Inc. All rights reserved.

Publication Date

2-16-2012

Publication Title

Journal of Physical Chemistry Letters

Volume

3

Issue

2

Number of Pages

463-467

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1021/jz201551e

Socpus ID

84857541359 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84857541359

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