Title

Quantitative Comparison Of Raman Activities, Sers Activities, And Sers Enhancement Factors Of Organothiols: Implication To Chemical Enhancement

Keywords

Extracellular matrix; Keratin 8; Metastasis; Plasminogen; Plasminogen activator; Prostate cancer

Abstract

Serum PSA, Gleason score, pathological stage, and positive surgical margins are currently used as predictors for disease recurrence. However, these criteria are less than precise in predicting disease outcome, with only 10% specificity at the 90% sensitivity level. Keratins are intermediate filament proteins that are contained within normal epithelia. However, human prostate cancer tissue shows differential immunohistochemical staining of keratin 8 (K8) when com-paredto normal prostate tissue. Our immunofluorescence and flow cytometry data show that K8 is also present on the cell surface of transformed prostate cancer cell lines. K8 is expressed at high levels on the surfaces of DU-145 and PC-3 cells but is expressed at comparatively lower levels on the surfaces of LNCaP cells, BPH-1 cells, and RWPE-1 cells. We hypothesize that extracellular K8 (eK8) present on epithelial prostate cancer cells plays an integral role in migration and in vivo dissemination. We found that K8 increased the rate of activityofplasmin approximatelyfivefold over a 48-h period. Functionally, K8 also enhanced the plasmin-mediated pro-teolysis of vitronectin, an important component of the prostate extracellular matrix. Taken together, our data show that K8 enhances the proteolytic activity of the plasminogen activation system, indicating that eK8 may be an important distinguishing marker in prostate cancer and progression. © Springer Science+Business Media, LLC 2012.

Publication Date

3-1-2012

Publication Title

Journal of Physical Chemistry Letters

Volume

3

Issue

3

Number of Pages

560-565

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1021/jz2016439

Socpus ID

84863232332 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84863232332

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