Title

Cell Therapy In The Heart

Keywords

DAB-1; Neural progenitor; NICD; Notch; Radial glia; Reelin

Abstract

We have previously reported the cross-talk between Reelin and Notch-1 signaling pathways, which are 2 major pathways that regulate brain development. We found that Reelin activated Notch-1 signaling, leading to the expression of brain lipid binding protein (BLBP) and the formation of radial glial cells in human neural progenitor cells (hNPCs). In the current study, we investigated the molecular mechanisms by which Reelin activates Notch-1. We show that Reelin-stimulated Notch-1 activation is dependent on Reelin signaling. The induction of Disabled-1 (Dab-1) tyrosine phosphorylation, and the subsequent activation of Src family kinases, were found to be essential steps for the activation of Notch-1 signaling by Reelin. Reelin treatment increased the interaction between Dab-1 and Notch-1 intracellular domain (NICD), and enhanced NICD translocation to the nucleus. This study advances our knowledge of the regulation of Notch-1 activation by Reelin signaling in hNPCs, as an approach to understanding cell fate determination, differentiation, and neurogenesis during brain development.

Publication Date

3-1-2012

Publication Title

Canadian Journal of Physiology and Pharmacology

Volume

90

Issue

3

Number of Pages

307-315

Document Type

Review

Personal Identifier

scopus

DOI Link

https://doi.org/10.1139/Y11-130

Socpus ID

84858026090 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84858026090

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