Title

An Organizational View Of Privatization: Is The Private Foster Care Agency Superior To The Public Foster Care Agency?

Keywords

atherosclerosis; imaging agents; macrophages; magnetic resonance imaging

Abstract

Objective-Myeloid-related protein (Mrp) 8/14 complex (is a highly expressed extracellularly secreted protein, implicated in atherosclerosis. In this study, we evaluated the feasibility of targeting Mrp in vivo through synthetic immuno-nanoprobes. Methods and Results-Anti-Mrp-14 and nonspecific IgG-conjugated gadolinium nanoprobes (aMrp-) were synthesized and characterized. Pharmacokinetics and vascular targeting via MRI of the formulations were assessed in vivo in high fat-fed apolipoprotein E deficient (ApoE -/-), ApoE -/-/Mrp14 (double knockout) and chow-fed wild-type (C57BL/6) mice. Bone marrow-derived myeloid progenitor cells were isolated from both ApoE -/- and double knockout mice, differentiated to macrophages, and were treated with LPS, with or without Mrp8, Mrp14, or Mrp8/14; conditioned media was used for in vitro studies. Mrp-activated cells secreted significant amounts of proinflammatory cytokines, which was abolished by pretreatment with aMrp-NP. We show in vitro that aMrp-NP binds endothelial cells previously treated with conditioned media containing Mrp8/14. MRI following intravenous delivery of aMrp-NP revealed prolonged and substantial delineation of plaque in ApoE -/- but not double knockout or wild-type animals. Nonspecific IgG-conjugated gadolinium nanoprobe-injected animals in all groups did not show vessel wall enhancement. Flow-cytometric analysis of aortic digesta revealed that aMrp-NP present in Ly-6G +, CD11b +, CD11c +, and CD31 + cells in ApoE -/- but not in double knockout animals. Conclusion-Targeted imaging with aMrp-NP demonstrates enhancement of plaque with binding to inflammatory cells and reduction in inflammation. This strategy has promise as a theranostic approach for atherosclerosis. © 2012 American Heart Association, Inc.

Publication Date

4-1-2012

Publication Title

Children and Youth Services Review

Volume

34

Issue

4

Number of Pages

851-858

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.childyouth.2012.01.016

Socpus ID

84862820874 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84862820874

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