Title

The Effects Of Instructor Fear Appeals And Moral Appeals On Cheating-Related Attitudes And Behavior Of University Students

Keywords

Asthenozoospermia; Azoospermia; Birefringence; ICSI; Polscope

Abstract

The ideal method for sperm selection during Intracytoplasmic sperm injection (ICSI) is still ill-defined. Identification of a viable spermatozoon amongst immotile spermatozoa for ICSI often becomes difficult. Ninety-six ICSI cycles were selected and divided into Group A (azoospermic, n=58) and Group B (complete asthenozoospermic, n=38). Oocytes having birefringent meiotic spindle and zona pellucida thickness <20μm were selected for ICSI. Groups A and B were further divided into A1, A2 and B1, B2, respectively, based on the type of ICSI performed. In Group A1, a motile spermatozoon with normal morphology was injected into a metaphase-II (M-II) oocyte. In Group B1, spermatozoon showing coiling of tail following modified hypo-osmotic swelling test was injected into M-II oocytes. In Groups A2 and B2, ICSI was performed by injecting a spermatozoan with birefringent head. Pronuclear morphology, fertilisation rate, embryo grading and pregnancy rate were assessed. ICSI outcome measures were better in Group A2 than in Group A1 but were statistically insignificant. However, significantly higher percentage of Z1 and Z2 zygotes, Grade I and Grade II embryos and pregnancy rate were observed in Group B2 as compared to Group B1. Selection of birefringent spermatozoa shows promising results in asthenozoospermic men and men undergoing testicular sperm aspiration or extraction before ICSI. © 2012 Blackwell Verlag GmbH.

Publication Date

5-1-2012

Publication Title

Ethics and Behavior

Volume

22

Issue

SUPPL.1

Number of Pages

196-207

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1080/10508422.2012.659603

Socpus ID

84861523233 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84861523233

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