Title

Performance Evaluation And Optimization Of Multiplex Pcrs For The Highly Discriminating Osu 10-Locus Set Y-Strs

Keywords

DYS448; DYS487; DYS488; DYS504; DYS576; DYS657; DYS685; DYS688; DYS703; DYS707; Forensic science; Microsatellites; Short tandem repeats; Y-chromosome

Abstract

In a previous study, a new set of Y-chromosome short tandem repeats, the OSU 10-locus set (MPM1 and MPM2), was shown to have a higher discrimination power when evaluated against the 10 SWGDAM loci on a common population panel. Here, we describe the optimization of the multiplex reactions using dye-labeled primers followed by performance evaluations. The loci exhibited high precision, human male specificity, reliability in different body fluids, high sensitivity, stability, and the ability to amplify nonprobative casework and mixture samples. Stutter for the all of the loci, with the exception of the highly polymorphic locus DYS688, was similar to that observed for autosomal loci. The results of the performance evaluations reinforced the utility of these loci. © 2011 American Academy of Forensic Sciences.

Publication Date

1-1-2012

Publication Title

Journal of Forensic Sciences

Volume

57

Issue

1

Number of Pages

52-59

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1111/j.1556-4029.2011.01910.x

Socpus ID

84855351124 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84855351124

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