Title

Predicting Detection Limits Of Enzyme-Linked Immunosorbent Assay (Elisa) And Bioanalytical Techniques In General

Abstract

The detection limit is one of the most important performance parameters for bioanalytical techniques. Here we present a generic method to estimate the detection limit of biomolecular assays based on a step-by-step analysis of the assay procedure. Enzyme-linked immunosorbent assay (ELISA) is used here as an example; however, much of the information presented in this article may be applied to other types of biomolecular assays and analytical techniques. A clear understanding of what affects the detection limit can help researchers to evaluate different bio-analytical techniques properly, and to design better strategies to optimize and achieve the best analytical performance. © 2013 The Royal Society of Chemistry.

Publication Date

12-9-2013

Publication Title

Analyst

Volume

139

Issue

2

Number of Pages

439-445

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1039/c3an01835k

Socpus ID

84890068615 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84890068615

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