Title

Four-Way Junction Formation Promoting Ultrasensitive Electrochemical Detection Of Microrna

Abstract

MicroRNAs (miRNAs) represent a class of biomarkers that are frequently deregulated in cancer cells and have shown a great promise for cancer classification and prognosis. Here, we endeavored to develop a DNA four-way junction based electrochemical sensor (4J-SENS) for ultrasensitive miRNA analysis. The developed sensor can be operated within the dynamic range from 10 aM to 1 fM and detect as low as 2 aM of miR-122 (∼36 molecules per sample), without PCR amplification. Furthermore, the 4J-SENS was employed to profile endogenouse hsa-miR-122 in healthy human and chronic lymphocyitc leukemia (CLL) patient serum, and the results were validated by qPCR analysis. © 2013 American Chemical Society.

Publication Date

10-15-2013

Publication Title

Analytical Chemistry

Volume

85

Issue

20

Number of Pages

9422-9427

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1021/ac402416z

Socpus ID

84886876486 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84886876486

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