Title

Temporal Patterns Of Tyrosine Nitration In Embryo Heart Development

Keywords

Development; Free radicals; Heart; Nitric oxide synthase; Nitrotyrosine

Abstract

Tyrosine nitration is a biomarker for the production of peroxynitrite and other reactive nitrogen species. Nitrotyrosine immunoreactivity is present in many pathological conditions including several cardiac diseases. Because the events observed during heart failure may recapitulate some aspects of development, we tested whether nitrotyrosine is present during normal development of the rat embryo heart and its potential relationship in cardiac remodeling through apoptosis. Nitric oxide production is highly dynamic during development, but whether peroxynitrite and nitrotyrosine are formed during normal embryonic development has received little attention. Rat embryo hearts exhibited strong nitrotyrosine immunoreactivity in endocardial and myocardial cells of the atria and ventricles from E12 to E18. After E18, nitrotyrosine staining faded and disappeared entirely by birth. Tyrosine nitration in the myocardial tissue coincided with elevated protein expression of nitric oxide synthases (eNOS and iNOS). The immunoreactivity for these NOS isoforms remained elevated even after nitrotyrosine had disappeared. Tyrosine nitration did not correlate with cell death or proliferation of cardiac cells. Analysis of tryptic peptides by MALDI-TOF showed that nitration occurs in actin, myosin, and the mitochondrial ATP synthase α chain. These results suggest that reactive nitrogen species are not restricted to pathological conditions but may play a role during normal embryonic development. © 2012 Elsevier Inc.

Publication Date

2-1-2013

Publication Title

Free Radical Biology and Medicine

Volume

55

Number of Pages

101-108

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.freeradbiomed.2012.10.535

Socpus ID

84871375040 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84871375040

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