Title

Responses Of Proenkephalin Peptide F To Aerobic Exercise Stress In The Plasma And White Blood Cell Biocompartments

Keywords

Buffy coat; Enkephalin-containing peptides; Exercise; Immune function; Opioid responses to exercise

Abstract

Proenkephalin Peptide F [107-140] is an enkephalin-containing peptide found predominantly within the adrenal medulla, co-packaged with epinephrine within the chromaffin granules. In vivo studies indicate that Peptide F has classic opioid analgesia effects; in vitro studies suggest potential immune cell interactions. In this investigation we examined patterns of Peptide F concentrations in different bio-compartments of the blood at rest and following sub-maximal cycle exercise to determine if Peptide F interacts with the white blood cell (WBC) bio-compartment during aerobic exercise. Eight physically active men (n = 8) performed sub-maximal (80-85% V̇O2Speak) cycle ergometer exercise for 30 min. Plasma Peptide F and WBC Peptide F immunoreactivity were examined pre-exercise, mid-exercise and immediately post-, 5-min post-, 15-min post-, 30-min post- and 60-min post-exercise and at similar time-points during a control condition (30 min rest). Peptide F concentrations significantly (p < 0.05) increased at 5 and 60 min post-exercise, compared to pre-exercise concentrations. No significant increases in Peptide F concentrations in the WBC fraction were observed during or after exercise. However, a significant decrease was observed at 30 min post-exercise. An ultradian pattern of Peptide F distribution was apparent during rest. Furthermore, concentrations of T cells, B cells, NK cells, and total WBCs demonstrated significant changes in response to aerobic exercise. Data indicated that Peptide F was bound in significant molar concentrations in the WBC fraction and that this biocompartment may be one of the tissue targets for binding interactions. These data indicate that Peptide F is involved with immune cell modulation in the white blood circulatory biocompartment of blood.© 2013 Elsevier Inc.

Publication Date

4-1-2013

Publication Title

Peptides

Volume

42

Number of Pages

118-124

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.peptides.2013.01.013

Socpus ID

84874639401 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84874639401

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