Title

Derivation Of Sensory Neurons And Neural Crest Stem Cells From Human Neural Progenitor Hnp1

Keywords

Cell differentiation; Human; Neural crest stem cell; Neural progenitor; Schwann cell; Sensory neuron; Stem cell

Abstract

Although sensory neurons constitute a critical component for the proper function of the nervous system, the in vitro differentiation of functional sensory neurons from human stem cells has not yet been reported. This study presents the differentiation of sensory neurons (SNs) from a human neural progenitor cell line, hNP1, and their functional maturation in a defined, in vitro culture system without murine cell feeder layers. The SNs were characterized by immunocytochemistry and their functional maturation was evaluated by electrophysiology. Neural crest (NC) precursors, as one of the cellular derivatives in the differentiation culture, were isolated, propagated, and tested for their ability to generate sensory neurons. The hSC-derived SNs, as well as the NC precursors provide valuable tools for developing in vitro functional systems that model sensory neuron-related neural circuits and for designing therapeutic models for related diseases. © 2013 Elsevier Ltd.

Publication Date

6-1-2013

Publication Title

Biomaterials

Volume

34

Issue

18

Number of Pages

4418-4427

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.biomaterials.2013.02.061

Socpus ID

84875600388 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84875600388

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