Title

Determination Of Polycyclic Aromatic Hydrocarbon Metabolites In Milk By A Quick, Easy, Cheap, Effective, Rugged And Safe Extraction And Capillary Electrophoresis

Keywords

Capillary electrophoresis; Metabolites; Milk; Polycyclic Aromatic Hydrocarbons

Abstract

We describe the first application of a quick, easy, cheap, effective, rugged and safe extraction technique to the CZE analysis of monohydroxylated metabolites of polycyclic aromatic hydrocarbons in milk. Complete resolution of 2-hydroxyfluorenene, 1-hydroxynaphthalene, 2-hydroxynaphthalene, 3-hydroxyphenanthrene, and 9-hydroxyphenanthrene was accomplished in 4 min of electrophoretic run. Limits of detection at the parts-per-billion were obtained with a single solvent (acetonitrile) for metabolite extraction and sample stacking. The small sample volume (1.2 mL) and the conservative usage of chemicals provided a simple and rapid procedure for the simultaneous extraction of numerous samples. Adding 4 min of electrophoretic run per sample, it should be possible to screen ten samples in approximately 1 h of analysis time. The nanoliter extract volume required for sample injection allows for further chromatographic usage and confirmation of positive samples. The unique electrophoretic pattern of the studied metabolites demonstrates the potential for the unambiguous determination of positional isomers with very similar chromatographic behaviors and undistinguishable mass fragmentation patterns. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Publication Date

7-1-2013

Publication Title

Journal of Separation Science

Volume

36

Issue

14

Number of Pages

2291-2298

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1002/jssc.201300250

Socpus ID

84880286661 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84880286661

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