Title

Caveolae-Mediated Endocytosis Of Conjugated Polymer Nanoparticles

Keywords

Conjugated polyelectrolytes; Conjugated polymer nanoparticles; Conjugated polymers; Endocytosis mechanism; Small interfering RNA delivery

Abstract

Understanding the cellular entry pathways of synthetic biomaterials is highly important to improve overall labeling and delivery efficiency. Herein, cellular entry mechanisms of conjugated polymer nanoparticles (CPNs) are presented. CPNs are intrinsic fluorescent materials used for various biological applications. While CPNs cause no toxicity, decreased CPN uptake is observed from cancer cells pretreated with genistein, which is an inhibitor of caveolae-mediated endocytosis (CvME). CvME is further confirmed by high co-localization with caveolin-1 proteins found in the caveolae and caveosomes. Excellent photophysical properties, non-toxicity, and non-destructive delivery pathways support that CPNs are promising multifunctional carriers minimizing degradation of contents during delivery. A detailed cellular entry mechanism of conjugated polymer nanoparticles (CPNs) is presented. Cancer cells pretreated with an inhibitor of caveolae-mediated endocytosis (CvME) exhibit decreased CPN uptake. High co-localization with caveolin-1 proteins found in the caveolae and caveosomes further confirms CvME of CPNs. Non-toxicity and non-destructive delivery pathways support that CPNs are promising carriers, minimizing content degradation during delivery. © 2013.

Publication Date

7-1-2013

Publication Title

Macromolecular Bioscience

Volume

13

Issue

7

Number of Pages

913-920

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1002/mabi.201300030

Socpus ID

84880703856 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84880703856

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