Title

Snp Analysis Using A Molecular Beacon-Based Operating Cooperatively (Oc) Sensor

Keywords

DNA four-way junction; Fluorescent probe; Molecular beacon; Nucleic acid detection; Single-nucleotide polymorphism

Abstract

Analysis of single-nucleotide polymorphisms (SNPs) is important for diagnosis of infectious and genetic diseases, for environment and population studies, as well as in forensic applications. Herein is a detailed description to design an "operating cooperatively" (OC) sensor for highly specific SNP analysis. OC sensors use two unmodified DNA adaptor strands and a molecular beacon probe to detect a nucleic acid targets with exceptional specificity towards SNPs. Genotyping can be accomplished at room temperature in a homogenous assay. The approach is easily adaptable for any nucleic acid target, and has been successfully used for analysis of targets with complex secondary structures. Additionally, OC sensors are an easy-to-design and cost-effective method for SNP analysis and nucleic acid detection. © 2013 Springer Science+Business Media, New York.

Publication Date

1-1-2013

Publication Title

Methods in Molecular Biology

Volume

1039

Number of Pages

81-86

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1007/978-1-62703-535-4_6

Socpus ID

84934440235 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84934440235

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