Title

Filament-Associated Tsga10 Protein Is Expressed In Professional Antigen Presenting Cells And Interacts With Vimentin

Keywords

Dendritic cell; Intermediate filament; Macrophage; TSGA10; Vimentin

Abstract

Testis-specific gene antigen 10 (TSGA10) encodes an 82-kDa protein expressed during development, and in testis and brain tissues. We report its expression in human monocyte-derived dendritic cells (DC) and macrophages in vitro and in murine spleen CD11c+ cells ex vivo. An interaction between DC/macrophage-derived TSGA10 and vimentin, as well as a few other major cytoskeletal proteins (e.g., actin-γ1), was identified by pull-down and mass spectroscopy assays. The interaction between TSGA10 and vimentin was further confirmed by immunoprecipitation and immunolocalisation in transfected RAW267 and HEK293 cell lines. TSGA10 formed filamentous structures in transfected COS-1 cells and was observed in cellular projections. We propose that TSGA10 could influence the function of antigen presenting cells (APC) via its interaction with cytoskeletal proteins such as vimentin. © 2010 Elsevier Inc.

Publication Date

9-2-2010

Publication Title

Cellular Immunology

Volume

265

Issue

2

Number of Pages

120-126

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.cellimm.2010.07.012

Socpus ID

77957016942 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/77957016942

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