Title

Targeted Nanoparticles For Enhanced X-Ray Radiation Killing Of Multidrug-Resistant Bacteria

Abstract

This paper describes a nanoparticle enhanced X-ray irradiation based strategy that can be used to kill multidrug resistant (MDR) bacteria. In the proof-of-concept experiment using MDR Pseudomonas aeruginosa (P. aeruginosa) as an example, polyclonal antibody modified bismuth nanoparticles are introduced into bacterial culture to specifically target P. aeruginosa. After washing off uncombined bismuth nanoparticles, the bacteria are irradiated with X-rays, using a setup that mimics a deeply buried wound in humans. Results show that up to 90% of MDR P. aeruginosa are killed in the presence of 200 μg ml-1 bismuth nanoparticles, whereas only ∼6% are killed in the absence of bismuth nanoparticles when exposed to 40 kVp X-rays for 10 min. The 200 μg ml-1 bismuth nanoparticles enhance localized X-ray dose by 35 times higher than the control with no nanoparticles. In addition, no significant harmful effects on human cells (HeLa and MG-63 cells) have been observed with 200 μg ml-1 bismuth nanoparticles and 10 min 40 kVp X-ray irradiation exposures, rendering the potential for future clinical use. Since X-rays can easily penetrate human tissues, this bactericidal strategy has the potential to be used in effectively killing deeply buried MDR bacteria in vivo. © The Royal Society of Chemistry.

Publication Date

1-21-2013

Publication Title

Nanoscale

Volume

5

Issue

2

Number of Pages

687-694

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1039/c2nr33154c

Socpus ID

84871744023 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84871744023

This document is currently not available here.

Share

COinS