Title

From Surface To Intracellular Non-Invasive Nanoscale Study Of Living Cells Impairments

Keywords

glyphosate; keratinocytes; living cells; mode-synthesizing atomic force microscopy; nanoscale resolution; non-invasive; subsurface imaging

Abstract

Among the enduring challenges in nanoscience, subsurface characterization of living cells holds major stakes. Developments in nanometrology for soft matter thriving on the sensitivity and high resolution benefits of atomic force microscopy have enabled detection of subsurface structures at the nanoscale. However, measurements in liquid environments remain complex, in particular in the subsurface domain. Here we introduce liquid-mode synthesizing atomic force microscopy (l-MSAFM) to study both the inner structures and the chemically induced intracellular impairments of living cells. Specifically, we visualize the intracellular stress effects of glyphosate on living keratinocytes skin cells. This new approach, l-MSAFM, for nanoscale imaging of living cell in their physiological environment or in presence of a chemical stress agent could resolve the loss of inner structures induced by glyphosate, the main component of a well-known pesticide (RoundUp™). This firsthand ability to monitor the cell's inner response to external stimuli non-destructively and in liquid, has the potential to unveil critical nanoscale mechanisms of life science. © 2014 IOP Publishing Ltd.

Publication Date

7-25-2014

Publication Title

Nanotechnology

Volume

25

Issue

29

Number of Pages

-

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1088/0957-4484/25/29/295101

Socpus ID

84903974022 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84903974022

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