Title

Novel Hydrophilic Bis(1,2,3-Triazolyl)Fluorenyl Probe For In Vitro Zinc Ion Sensing

Keywords

1,2,3-triazolyl-fluorene; binding constant; fluorescent probe; metal sensing; two-photon microscopy bioimaging; zinc ion sensing

Abstract

A hydrophilic bis(1,2,3-triazolyl)fluorene derivative was synthesized as a multi-photon-absorbing, zinc-ion-sensing fluorescent probe. The fluorescence response was approximately five-fold greater in presence of Zn2+, resulting in a large binding constant (1 × 109) for a 1:2 ligand to zinc complex. A four-fold increase in the two-photon absorption cross section was achieved upon binding Zn2+. In vitro two-photon fluorescence microscopy imaging revealed a significant fluorescence increase upon introduction of Zn2+ into HeLa cells and reversible Zn 2+ binding, demonstrating the potential of this probe for zinc ion sensing. © 2010 American Chemical Society.

Publication Date

11-24-2010

Publication Title

ACS Applied Materials and Interfaces

Volume

2

Issue

11

Number of Pages

2978-2981

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1021/am100719v

Socpus ID

79151481873 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/79151481873

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